Two-Way ANOVA Calculator for Independent Measures
This calculator performs a two-way analysis of variance (ANOVA) for independent measures, testing the effects of two independent variables (factors) on a dependent variable, as well as the interaction effect between the two factors.
When to Use
Use two-way ANOVA when you want to examine:
- Main Effect of Factor A: Whether there are statistically significant differences between the levels of the first independent variable
- Main Effect of Factor B: Whether there are statistically significant differences between the levels of the second independent variable
- Interaction Effect: Whether the effect of one factor depends on the level of the other factor (e.g., does the effect of treatment differ for males vs. females?)
Example Research Questions
- Does test performance differ by gender (Factor A) and teaching method (Factor B)? Is there an interaction?
- Does plant growth vary by fertilizer type (Factor A) and sunlight exposure (Factor B)?
- Does customer satisfaction differ by store location (Factor A) and time of day (Factor B)?
Requirements
- Two independent categorical variables (factors), each with 2+ levels
- One continuous dependent variable (interval/ratio scale)
- Independent observations within and between groups
- Approximately normal distribution within each cell (combination of factor levels)
- Homogeneity of variances across all cells (equal variances)
Hypotheses
Factor A (Rows)
H₀: All levels of Factor A have equal means (μₐ₁ = μₐ₂ = ... = μₐₖ)
H₁: At least one level of Factor A has a different mean
Factor B (Columns)
H₀: All levels of Factor B have equal means (μᵦ₁ = μᵦ₂ = ... = μᵦⱼ)
H₁: At least one level of Factor B has a different mean
Interaction (A × B)
H₀: There is no interaction between Factor A and Factor B
H₁: There is an interaction between Factor A and Factor B
ANOVA Table Components
SS (Sum of Squares): Measures variation for each source
df (Degrees of Freedom): Number of independent values for each source
MS (Mean Square): SS divided by df (variance estimate)
F-ratio: MS effect / MS error (within cells)
p-value: Probability of observing this F-ratio if H₀ is true
Effect Size Interpretation
Eta-squared (η²): Proportion of total variance in the dependent variable explained by each factor. Values range from 0 to 1.
- η² ≈ 0.01: Small effect
- η² ≈ 0.06: Medium effect
- η² ≈ 0.14 or higher: Large effect
Partial Eta-squared: Proportion of variance explained excluding variance from other factors. Generally larger than eta-squared.
Assumptions Check
Before interpreting results, check these assumptions:
- Independence: Observations should be independent. Violations require repeated measures ANOVA or multilevel modeling.
- Normality: Use the Shapiro-Wilk test or Q-Q plots to check normality within each cell. With large samples, ANOVA is robust to violations.
- Homogeneity of Variance: Use Levene's test. If violated, consider Welch's ANOVA or data transformation.
Interpreting Interaction Effects
A significant interaction means the effect of one factor depends on the level of the other factor. For example, a treatment might work well for one group but not for another.
Important: If the interaction is significant, interpret it first before examining main effects. Main effects can be misleading when there's a significant interaction (simple main effects analysis may be needed).